This project is aimed to develop an effective method to prevent IHHNV infection in the shrimp by employing specific double-stranded RNA (dsRNA). The objectives include:
1. To determine infectivity of IHHNV in shrimp by laboratory injection.
2. To construct dsRNA from different genes of IHHNV genome.
3. To determine the potency of each IHHNV-specific dsRNA to inhibit viral replication in shrimp
4. To develop dsRNA-directed gene silencing as a method for effective prevention of viral diseases in shrimp