1. To investigate the effect of LPS on AMPK activation, its underlying mechanism and its involvement in the regulation of endocrine function and intestinal barrier function in IEC.
2. To investigate the effect of TNF? on potentiating AMPK-inhibiting activity of LPS, its underlying mechanisms and its contribution to the regulation of endocrine function and intestinal barrier function in IEC.
3. To investigate the effect of CaMKK? stimulation on alleviating the negative effect of LPS and TNF? on endocrine function and intestinal barrier function in IEC.
4. To identify membrane receptors whose engagement can lead to AMPK activation and consequent alleviation of the inflammation-induced suppression of gut hormone production and intestinal barrier disruption.
5. To investigate effects of CaSR, GPR39, GPR120 and TLR-5 agonist on ameliorating metabolic abnormalities in a mouse model of high-fat diet-induced obesity and insulin resistance.
