Development of sandwich enzyme-linked immunosorbent assay using monoclonal and polyclonal antibodies against Cathepsin L (CatL) for diagnosis of fasciolosis caused by F. gigantica
1 To produce and characterize MoAb and PoAb specifically against rCatL of F. gigantica.
2 To purify the MoAb and PoAb against rCatL of F. gigantica.
3 To develop a monoclonal antibody (MoAb)-based sandwich ELISA for the detection of circulating CatL in serum of experimentally and naturally infected animals with fasciolosis caused by F. gigantica.