1. To formulate diclofenac sodium-loaded trimethylchitosan (TMC) nanoparticles for ophthalmic use.
2. To study effect of formulation compositions on physicochemical properties of diclofenac sodium-loaded TMC nanoparticles.
3. To evaluate potential for being an eye irritant of diclofenac sodium-loaded TMC nanoparticles.
4. To study in vivo ophthalmic absorption and determine pharmacokinetic parameters of diclofenac sodium in aqueous humor from using diclofenac sodium-loaded TMC nanoparticles.