Identification of a reactive chlorine species-specific transcriptional regulator and its regulon in a human pathogen Pseudomonas aeruginosa
หัวหน้าโครงการ
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อดิศักดิ์ ร่มแสง
ทีมวิจัย
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อดิศักดิ์ ร่มแสง
หัวหน้าโครงการ
ศกรณ์ มงคลสุข
นักวิจัยที่ปรึกษา
วันที่เริ่มโครงการ
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2 พ.ค. 2559
วัตถุประสงค์
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The main objective is to identify a novel gene encoding a reactive chlorine species-specific transcriptional regulator in a human pathogen P. aeruginosa PAO1 and to characterize its regulon and mechanisms for sensing and responding to RCS-generated agents and the role in bacterial pathogenicity. In this study, the objective will be divided into 8 sub-objectives as follow:
2.1. To identify RclR-homologous genes in P. aeruginosa PAO1 genome and to construct the gene-deleted mutant
2.2. To analyze physiological roles of these genes against several stress-generated agents including reactive oxygen species (ROS) and reactive chlorine species (RCS)
2.3. To construct the functional gene expression vector for complementation assay
2.4. To determine the key amino acids responsible for the phenotype of the mutants
2.5. To investigate a gene expression profile under stress inducing conditions
2.6. To identify the RclR-targeted genes by using specific gene approach
2.7. To characterize the regulatory elements for transcriptional control
2.8. To investigate the role of these genes in bacterial pathogenicity
