2.1 To investigate the cytotoxic, apoptosis-inducing and anti-proliferative effects of cucurbitacin B against human CCA cells and its molecular mechanism of actions
2.2 To determine the anti-metastasis effect of cucurbitacin B on CCA cells and the molecular mechanism mediating its effect
2.3 To determine the effect of cucurbitacin B on upstream signaling cascades regulating survival, growth and metastasis of CCA cells including JAK/STAT3, NF-?B, FAK, PI3K/Akt and ERK1/2 pathways
2.4 To investigate the in vivo anti-cancer effect of cucurbitacin B using xenograft mouse model
