1 To determine the levels of ROS in CCA cells in vitro and in vivo under high and normal glucose conditions.
2 To investigate the effect of ROS on CCA progression under high glucose condition in CCA cell lines and inoculated mice.
3 To determine the expression level of oxidative stress related genes in CCA cell lines, CCA inoculated mice and CCA patients under high and normal glucose conditions.
4 To verify the molecular mechanism underlying oxidative stress induces CCA progression under high glucose condition.
