The ER transmembrane J-proteins are involved in formation of ER-mitochondria junction
หัวหน้าโครงการ
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ภัทราวุธ โสภา
ทีมวิจัย
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ภัทราวุธ โสภา
หัวหน้าโครงการ
ปิยะรัตน์ โกวิทตรพงศ์
นักวิจัยที่ปรึกษา
วันที่เริ่มโครงการ
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2 พ.ค. 2561
วัตถุประสงค์
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Aim 1 Identify the roles of JB12/RMA1 in maintaining mitochondria-associated membranes (MAM). The cytosolic face of the ER membrane is a place for intracellular signaling facilitating communication between ER and cytosol. As mentioned above, JB12 protrudes its J-domain into the cytosol, it might be possible that JB12 is involved in protein assembly/disassembly events such as modulation of MAM occurring on the cytoplasmic face of the ER. Then, degradation of JB12 during severe stress might be a mechanism that cell use for controlling communication between ER and mitochondria.
Aim 2 How does perturbed mitochondria communicate with ER?
Recent research focus in cancers has been centered on mitochondria as a crucial factor for cancer cell adaptation and survival. Mitochondria are a cellular powerhouse as well as a hub for pivotal cellular signaling such as calcium and apoptosis. The disturbance of mitochondria homeostasis triggers selective protein quality control mitophagy that eliminates the damaged or dysfunctional mitochondria. Perturbation of ER homeostasis, specifically caused ER stress, in non-recoverable situation, can lead to apoptosis via mitochondrial pathway. Although the signaling from the disturbed ER to mitochondria has been well characterized, how direct disruption of mitochondrial quality control affects ER is still yet to be understood. Therefore, this aim main focus is to observe the transmembrane chaperones and ERAD folding factors during homeostasis perturbation of mitochondria
