1 To identify the amino acid residues in the glycone binding pocket of beta-glucosidases that are important for glycone specificity by multiple site-directed mutagenesis.
2 To identify the amino acid residues in the glycone binding pocket of beta-glucosidases that are important for glycone specificity by directed evolution.
3 To identify the amino acid residues in the aglycone binding pocket of beta-glucosidases that are important for aglycone specificity by multiple site-directed mutagenesis.
4 To use dalcochinase mutants to synthesize useful alkyl glucosides by reverse hydrolysis reactions.
