This research work is an attempt to study the origin of colloidal behavior of NR latex and the components which act to colloidal system, together with topology and rubber membrane properties using microscopic techniques. The open structural questions remain unsolved that whether the membrane surrounded the rubber particles is a kind of mono- or bi-layer, and whether the particles are surrounded by a complete membrane or by an incomplete patchwork of membrane fragments. The arrangement of phospholipids and proteins at the surface of rubber particles by AFM technique is of interested in order to image rubber particle morphology and to determine the mechanical performance by nano-indentation. In addition, by selective fluorescence labeling of proteins as well as phospholipids, these constituents could be identified. The combination of AFM with fluorescence microscopy was applied to get new insights for the rubber film formation mechanism.
