1. To determine the localization and distribution of GnRH protein and kisspeptin protein in hypothalamus of pre-puberty and post-puberty swamp buffalo heifer.
2. To determine the localization and distribution of mRNA Kiss-1 gene in hypothalamus of pre-puberty and post-puberty swamp buffalo heifer.
3. To determine the interaction of KISS1R and GnRH neurons in hypothalamus of swamp buffalo heifer.