2.1 To apply expression profiles from GEO datasets, NCBI using Connection Up- and Down-Regulation Expression Analysis of Microarrays (CU-DREAM) and to determine genes up- and down- regulate hypomethylation process in replicative aging using CU-DREAM eXtension (CU-DREAMX) statistical analysis, a program for the study a relationship between two microarray data, to perform in laboratory study
2.2 To elucidate the methylation status and expression profiles in replicative cellular aging in laboratory study
2.3 To determine genes up- and down- regulate hypomethylation process in replicative aging in laboratory study
2.4 To generate siRNAs to knock down gene expression by the expression of down regulated gene promoting down regulation of hypomethylated genes related replicative aging (gene group A) and of up regulated gene promoting up regulation of hypomethylated genes related replicative aging (gene group B) in aging cells to reverse aging in laboratory study
2.5 To generate recombinant pEGFP-C2-gene group A plasmid and recombinant pEGFP-C2-gene group B plasmid in aging cells to reverse aging in laboratory study
