1 To determine patterns and levels of IgE and subclass of IgG responses to allergenic proteins derived from Dermatophagoides farinae, D. pteronyssinus, Malassezia pachydermatis and Staphylococcus psuedointermedius in serum of atopic dogs and non-atopic dogs
2 To characterize allergenic proteins extracted from each allergen by two-dimensional gel electrophoresis followed by western blotting
3 To characterize allergenic proteome by LC-MS/MS and/or peptide sequencing