Development of direct sandwich enzyme-linked immunosorbent assay using monoclonal and polyclonal antibodies specifically against major surface proteins (MSPs) for diagnosis of anaplasmosis caused by Anaplasma marginale
2.1 To analyze, isolate and characterize the initial body (IB) antigens or recombinant major surface protein of A. marginale (rAmMSP).
2.2 To produce and characterize MoAb and PoAb specifically against recombinant A. marginale major surface protein (rAmMSP).
2.3 To purify the MoAb and PoAb specifically against recombinant A. marginale major surface protein (rAmMSP).
2.4 To develop the direct sandwich enzyme-linked immunosorbent assay (direct sandwich ELISA) for the detection of the circulating major surface protein (MSP) antigens in serum of naturally infected animals with anaplasmosis caused by A. marginale.
