1. To determine the effectiveness of the new strategy in prenatal control for severe thalassemia syndrome
2. To identify the effectiveness of non-invasive approach for early diagnosis of fetuses at risk of homozygous thalassemia-1
3. To determine the normal reference range of fetal hematology and fetal cardiology
4. To determine cordocentesis associated complications in real practice
5. To evaluate the effectiveness of fetal cardiac defect using STIC technology
6. To evaluate the efficacy of sonographic markers in the first trimester in predicting fetal homozygous alpha-thalassemia-1 in couples at risk
7. To assess fetal cardiac function in differentiating high and low-output heart failure
8. To determine the effectiveness of maternal serum biochemical markers (PIGF, sFlt-1 and PIGF/sFlt-1 ratio) for fetal anemia and fetal homozygous alpha thalassemia-1 disease detection.
9. To determine the effectiveness of fetal serum biochemical markers (cTnT and NT-proBNP) for fetal anemia detection.
