1. To determine the cytotoxic effect of aqueous and ethanolic extracts of C. formosum leaves on CCA cells and their molecular mechanism of actions
2. To investigate the effects of C. formosum crude extracts on CCA cell cycle and proteins involved in cell cycle regulation
3. To examine whether C. formosum crude extracts suppress the activation of JAK/STAT signaling pathway and STAT-regulated genes such as Bcl-2, Bcl-xL, survivin, VEGF, ICAM, etc. in CCA cells
4. To determine whether C. formosum crude extracts inhibit metastatic potential of CCA cells and whether the effect is mediated via JAK/STAT signaling pathway
5. To fractionate of the extracts for active constituents to enrich their activities
6. To study the anticancer effect of the active compounds in C. formosum extracts
