1 To determine the expression profile of tumor specific glycolytic enzymes?namely GLUT1, HK2, PKM2, LDHA?in CCA cell lines and CCA patient tissues.
2 To investigate the association between expression levels of GLUT1, HK2, PKM2, LDHA and clinicopathological findings/survival of CCA patients.
3 To verify the significant roles of the particular glycolytic enzymes on proliferation, motility, apoptosis and energy production of CCA cell lines and inoculated mice.
