Part 1: Simultaneous detection of canine infectious respiratory disease (CIRD) associated viruses by a multiplex reverse transcription polymerase chain reaction (mRT-PCR) assay
Objective
1. To develop the diagnostic tool for viral associated pathogens of CIRD by the simplex and multiplex reverse transcription polymerase chain reaction (mRT-PCR) with sensitivity and specificity comparison
3. To establish disease surveillance each particular virus for epidemiological monitoring the potentially air-borne zoonoses from animals to humans
3. To investigate the prevalence and genetic diversity of currently circulating CIRD associated viruses in Thailand and compare with the previous known sequences deposited in GenBank
Part 2: The metagenomic analyses of viruses isolated from respiratory samples of sick dogs with unknown causes of respiratory illness.
Objectives
1. To confirm the negative results of both simplex and multiplex PCR, performing a standardization of such methods.
2. To discover the novel strain of virus or novel virus that can be or not associated with respiratory illness in dogs.
3. To monitor the emerging virus which is possible to transmit to other animals and humans, alerting to public health concerns.
