1. To establish a system-wide transcriptional profile of plants subjected to ambient temperature changes, which will serve as a platform for investigating global and local regulatory mechanisms of temperature transcriptomes.
2. To elucidate the interplay between temperature-responsive phenotype and genetic effect using hybrid plants as a model, and identify differentially expressed genes between hybrids and parents.
3. To design and construct an analytic pipeline for next-generation sequencing (NGS) data from publicly available computer codes and applications, which can also be adapted and implemented on other genomes in the future.